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82 lines
3.5 KiB
Markdown
82 lines
3.5 KiB
Markdown
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# Morning Push Checklist – 2025-05-07
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## Objective
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Deliver a **zero-friction boot experience** for students: open Codespace ➜ talk to Lab Agent immediately, with no extra setup. All necessary secrets, tools, and docs must already be in place.
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---
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## 1. Infrastructure / Dev-Ops Tasks (NEW)
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- [ ] **Pre-build Dev Container**
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- Configure `devcontainer.json` → `features` & `updateContentCommand` so that Python deps, GitHub CLI, and Agent embeddings are built during Codespaces prebuilds.
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- Verify a fresh Codespace reaches "Agent ready" in <2 min.
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- [ ] **Centralise `OPENAI_API_KEY` secret**
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- Add repo-level Codespaces secret `OPENAI_API_KEY` (owner only). Students inherit runtime access, cannot view value.
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- Document in README > _Secrets are pre-loaded; no action required_.
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- [ ] **README Quick-Start for Students**
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- Replace multi-step environment instructions with a 3-step "Open ➜ Wait ➜ Chat" section.
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- Include GIF / screenshot.
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- [ ] **Automated Issue Logging**
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- Implement small Python hook (`Agent/hooks/post_issue.py`) to append newly created GitHub issues to `TASKS.md` and `ISSUES_LOG.md`.
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- Add instructions in `Lab Agent Guide`.
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- [ ] **GitLens Integration**
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- Add `.vscode/settings.json` to enable GitLens "Worktree Issues" view and pin lab-repo issues by default.
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- [ ] **CI Smoke Test** (optional)
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- GitHub Action that boots Codespace headless and runs `Agent/agent_runner.py --healthcheck`.
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---
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## 2. Carried-Over Wet-Lab Tasks (from 2025-05-06)
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These remain **open** and must be tracked alongside dev tasks.
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- [ ] Thaw HEK293T cells and check viability
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- [ ] Prepare transfection master mixes
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- [ ] siRNA targeting Ybx1
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- [ ] Control siRNA
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- [ ] Set up reverse transfection in 24-well plates
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- [ ] Label all plates and wells
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- [ ] Prepare Lipofectamine RNAiMAX and Opti-MEM mixtures
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- [ ] Add siRNA to appropriate wells
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- [ ] Add cells to all wells
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- [ ] Place plates in 37 °C incubator with 5 % CO₂
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- [ ] Update experiment status in repository
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- [ ] Upcoming Day 3 tasks (May 8) – verify knockdown & start actinomycin D time course
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- [ ] Materials check: Actinomycin D, RNA extraction kits, qPCR primers
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---
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## 3. GitHub Issues to Create (assign to @james-m-jordan)
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| # | Title | Labels |
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|---|-------|--------|
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| 1 | Pre-build Dev Container for instant Agent boot | infra, high-priority |
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| 2 | Add repo-level OPENAI_API_KEY secret & document | infra, security |
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| 3 | Rewrite README Quick-Start for students | docs |
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| 4 | Implement automated ISSUE → TASKS.md hook | tooling |
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| 5 | Add GitLens default issue view configuration | tooling |
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| 6 | CI: Codespace headless smoke test | ci |
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| 7 | [Wet-Lab] Complete HEK293T thaw & viability check | lab |
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| 8 | [Wet-Lab] Prepare Ybx1 & control siRNA mixes | lab |
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| … | _Continue one issue per checklist bullet above_ | |
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> **How to batch-create:**
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> ```bash
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> gh issue create -t "Pre-build Dev Container for instant Agent boot" -b "See morning_push_2025-05-07.md#1" -a james-m-jordan -l infra,high-priority
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> # Repeat for each row or use gh api to script.
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> ```
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---
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## 4. Deliverables for May 7 End-of-Day
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- Updated **README.md** (student quick-start)
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- Merged PR implementing dev-ops tasks 1–2
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- All GitHub issues above created and assigned
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- `ISSUES_LOG.md` scaffold committed
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---
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## 5. Review & Next Steps
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1. Mid-day stand-up: confirm secret injection works in fresh Codespace.
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2. Evening: demo 60-second "clone → chat" workflow with new student account.
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3. Plan May 8 push: continue lab experiment tracking + smoke test results.
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---
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_Authored automatically by Lab Agent on 2025-05-07_
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