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26 lines
1.2 KiB
YAML
26 lines
1.2 KiB
YAML
title: mRNA stability measurement after Ybx1 knockdown
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description: Investigation of mRNA stability changes in selected genes after Ybx1 knockdown, using siRNA and actinomycin D to assess post-transcriptional regulation
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date_started: 2025-05-06
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lead: Dr. Jim Jordan
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team_members:
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- Dr. Jim Jordan
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- Jack Zhao
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parent_project: Post-transcriptional regulation by Ybx1
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associated_protocols:
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- Ybx1 knockdown mRNA stability assay
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experiments:
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- TBD
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notes: |
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This subproject aims to determine if Ybx1 knockdown influences the stability of mRNAs,
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particularly those with known or suspected post-transcriptional modifications (m5C or m6A).
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Specific aims:
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1. Establish efficient Ybx1 knockdown using siRNA
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2. Measure half-lives of selected target mRNAs in control vs. Ybx1 knockdown conditions
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3. Identify genes most affected by Ybx1 depletion
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4. Correlate mRNA stability changes with RNA modifications if possible
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Initial target genes include Myc, p53, and other transcripts with known post-transcriptional regulation.
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This experiment will employ actinomycin D to block transcription, followed by time-course
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sampling and RT-qPCR to measure the decay rates of specific mRNAs. |