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19 lines
516 B
YAML
19 lines
516 B
YAML
name: Cell Staining Protocol
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id: PROT-0001
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description: Protocol for immunofluorescence staining of cells
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author: Alice Smith
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created: 2025-05-05
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version: 1.0
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materials:
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- Antibody: Anti-XYZ (1:500 dilution)
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- Stain: DAPI
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- Buffer: PBS 1X
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steps:
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- "Fix cells with 4% PFA for 10 minutes."
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- "Wash 3x with PBS."
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- "Add primary antibody (Anti-XYZ) for 1 hour at RT."
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- "Wash 3x with PBS."
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- "Add DAPI stain for 5 minutes."
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- "Wash and image."
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notes: |
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This protocol is derived from Doe et al. 2023. |