docs/aims.md

1.6 KiB

  1. Ybx1 in adipogenesis
    1. Identifying YBX1-CEBPA-cBAF interactions in early, middle, late 3T3-L1 adipogenesis LO
      1. Are we seeing this in all cell types?
    2. YBX1 in adipogenic 3T3 metabolism ?
    3. Loss of SMARCD2, SMARCE1, and YBX1 in differentating 3T3 cells CJ
  2. cBAF-CEBPa regulating mir-101 ?
  3. Ybx1 in metabolic reprogramming of hepatocytes
    1. PPARg, CEBPa, CEBPb, SMARCD2, SMARCE1, BRG1
    2. Posttranscriptional regulation of lipogenic genes by Ybx1
    3. YBX1 INS1 repression
      1. All cells except pancreatic beta cells?
        1. Absent in pancreatic beta cells?
          1. If so, how?
        2. Targeting YBX1 in certain types of Type 1 diabetes mellitus
    4. YBX1-CEBPa-cBAF in lipid-exposed hepatocytes multiomics
      1. mRNAseq
      2. ATACseq
      3. ChIPseq:
        1. YBX1,
        2. BRG1,
        3. CEBPa,
        4. (and CEBPb).
  4. Using artificial intelligence to enhance biological insights
    1. Geo stacking app ER
    2. Transregulator-ATAC pattern finder ?
    3. Increase read mapping speed ?
    4. Lab agent JJ
  5. Regulation of Ybx1 cyto-nuclear translocation
  6. Metabolic regulation of gene expression
    1. Different nutrient scenarios
    2. Insulin, inhibit beta-oxidation, inhibit glycolysis, ATACseq
  7. Environmental affects on hepatocyte cell fate
    1. KLFs
    2. HepaRG
  8. Mice
  9. Miscellaneous
  10. Spheroids/Organoids
  11. Robot
  12. Directional RNAseq
  13. DuoLink
  14. FA Uptake
  15. Proliferation
  16. Lipidomics
  17. Oxylipins
  18. CelSeq time series
  19. In vitro cytonuclear proteomics
    1. Epigenetic memory
      1. Mismatch