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docs/Protocols/BSA-OAPA Treatment Protocol v2.md

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#treatment Treatment Setup:

  • Final fatty acid concentration: 0.125 mM for both BSA-OA and BSA-PA.
  • Medium: Complete media with fatty acid-BSA conjugates.

Steps:

  1. Prepare BSA-fatty acid solutions separately.
  2. Dilute to final working concentrations in complete media.
  3. After 24 h in siRNA, treat cells for 48 h.