docs/Protocols/Preparation of Complete RPMI.md

Preparation of Complete RPMI

For a 500-ml Bottle

1. Sterile transfer ~51 ml of RPMI media into a sterile 50-ml tube.

   • Label: "PLAIN RPMI"

2. To the remaining 449 ml of media, add:

   • 50 ml Fetal Bovine Serum (FBS)
   • 1 ml Penicillin-Streptomycin (PS)

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