docs/Protocols/Preparation_of_Complete_DME_F12_ITSD.md

Preparation of Complete DME/F-12 ITSD

For a 500-ml Bottle

1. Sterile transfer ~55 ml of DME/F-12 media into a sterile 50-ml tube.

   • Label: "PLAIN DME/F-12"

2. To the remaining 445 ml of media, add:

   • 50 ml Fetal Bovine Serum (FBS)
   • 5 ml Insulin-Transferrin-Selenium (ITS)
   • 5 µl 10 mM dexamethasone
   • 1 ml Penicillin-Streptomycin (PS)

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