docs/Protocols/Seeding 96-Well Plate Protocol.md

Before You Begin

• Sterilize all materials with 70% ethanol.
• Complete plate layout and siRNA calculations beforehand.

Protocol

1. Open sterile 96-well TC plate (clear for brightfield / black for fluorescence).
2. Dilute 9 ml of cell suspension with 14 ml complete media.
3. Swirl and pour into a sterile 25-ml basin.
4. Using a multi-channel pipet:
   • Seed 100 µl per well.
5. Mix cell suspension periodically while seeding.
6. Aspirate leftovers and clean up.

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