docs/Protocols/Seeding Based on Confluence.md

For 6-Well Plates

1. Start with a 90–100% confluent 25 cm^3 flask of cells.
2. Aspirate media and wash with 10 ml sterile PBS.
3. Add 1 ml Trypsin, coat, incubate ~5 min at 37°C.
4. Dislodge cells, add 9 ml complete media (10% FBS + 1% PS).
5. Mix gently, pipet 350 µl cell suspension into each well.
6. Add 1600 µl complete media to each well.
7. Incubate 24–48 hours.

For 24-Well Plates

• Pipet 200 µl** cell suspension into each well.
• Top up with 800 µl media as needed.

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