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docs/Protocols/SpectraMax Plate Reader Protocol.md

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**

Created: 03/22/25JJ

  1. Open the SpectraMax software on the computer.

  2. Click Protocol > New Experiment

  3. Click the gear above the plate grid to adjust settings.

  4. For BCA:

  5. Absorbance mode

  6. Wavelengths = 562 nm

  7. Paint the wells you want to measure

  8. 96-well, clrbttm

  9. No shaking

  10. Fast Read is fine

  11. Click open/close plate reader button

  12. Insert your plate with H1 in the bottom-left corner.

  13. Press Read

  14. Copy paste the grid to excel

  15. Save excel file to a USB or to the hard drive and then transfer it off the core facility computer.

**